Sequencing of soy oil and soy lecithin allergens

• Published in: Journal of allergy and clinical immunology Vol. 113; no. 2; p. S152
• Main Authors: Errahali, Y., Kanny, G., Morisset, M., Cren-Olive, C., Rolando, C., Metche, M., Moneret-Vautrin, D.A., Nicolas, J.P., Fremont, S.
• Format: Journal Article
• Language: English
• Published: St. Louis Mosby, Inc 01.02.2004; Elsevier Limited
• ISSN: 0091-6749; 1097-6825
• DOI: 10.1016/j.jaci.2003.12.553

It has been shown that soy oil and soy lecithin may contain allergens. The aim of the present study is to characterize, by means of the physico-chemical and immunological methods, the allergenic proteins of soybean seed transferred to soy oil and soy lecithin during industrial refining. Extraction of proteins was conducted using a buffer solution. The identification of allergens used specific IgE from a soybean allergic patient. Bi-dimensional electrophoresis and sequencing from N and C terminals were used to characterize the allergenic proteins. Two allergenic proteins present in soybean seed, soy oil and soy lecithin have been isolated and identified: one 56 kDa non glycosylated allergen, whose isoelectric point of 5.4 has been identified as soybean β-Amylase (7S). One 20 kDa non glycosylated allergen, whose isoelectric point of 4.6 has been identified as Kunitz Trypsin inhibitor [Glycine max] (2S). We have sequenced two allergenic proteins present as hidden allergens in soy oil and soy lecithin. It is to note that these highly refining-resistant proteins are not the main proteins of soybean seed.