153 : HMGB1 and TNF-alpha in an experimental model of acute lung injury (ALI) under protective conventional mechanical ventilation (CMV)

• Published in: Cytokine (Philadelphia, Pa.) Vol. 63; no. 3; p. 279
• Main Authors: Kurokawa, Cilmery Suemi, Fioretto, José Roberto, de Araújo Júnior, João Pessoa, Pires, Rafaelle Batistella, Moraes, Marcos A, Klefens, Susiane O, Moretto, Maria Regina, Teixeira Fortes, Cristina Maria, Bonatto, Rossano Cesar, Carpi, Mario Ferreira
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 01.09.2013
• Subjects: compliance; grants; inflammation; macrophages; rabbits; tumor necrosis factor-alpha
• ISSN: 1043-4666; 1096-0023
• DOI: 10.1016/j.cyto.2013.06.156

Recently, HMGB1, a macrophage cytokine and nonhistone chromatin-associated protein, has been associated with sustained inflammatory response and production of cytokines in lung injury. Therefore, the aim of this study was to measure HMGB1 and TNF-alpha levels in bronchoalveolar lavage from rabbits with severe acute lung injury (ALI) induced by tracheal infusion of warm saline. Methods: Thirty rabbits were instrumented and randomly assigned into two groups: (1) animals with no ALI (Control Group-CG; n=15); and (2) animals with severe ALI (Severe Group-SG; n=15). The groups were ventilated during 4h with CMV. After ventilation, bronchoalveolar lavage fluid (BAL) was collected so that HMGB1, TNF-alpha levels and percentage of white cell were determined. Left lung was collected for histological analysis and confirmation of lesion. Results: Lung injury decreased pulmonary compliance (SG before: 1.86±0.576>SG after: 0.67±0.24) and oxygenation (PaO2 – SG before: 427.92±89.90>SG after: 68.18±19.08mmHg). Higher levels of HMGB1 were found in SG group as compared to CG group [SG: median-62 (p25–p75=20–79)>CG: 15 (13–25) ng/mL; p=0.035] and TNF-alpha level was similar to HMGB1 level in SG and CG groups [SG: 1078 (381–1224)>CG: 45 (25–86) pg/mL; p⩽0.001]. HMGB1 and TNF-alpha showed inverse correlation with oxygenation and positive correlation between them. Neutrophil percentage and histological score were higher in SG than CG (p=0.001 and p=0.0032, respectively). Conclusions: HMGB1 and TNF-alpha are found in alveolar space and correlation is found between them and with oxygenation in this experimental model. Fapesp grants: 2011/15144-2.