Plant Regeneration of Iris koreana Nakai through Organogenesis for Ex-situ Conservation

• Published in: Journal of forest and environmental science , 37(4) pp. 304 - 308
• Main Authors: 배기화, 윤이슬, Ji-Sun Jung, Chan-Beom Kim, Hye-Won Kim, 홍용식, Min-Kyeong Oak, 김학구, Ju-Hui Lee
• Format: Journal Article
• Language: English
• Published: 산림과학연구소 31.12.2021
• Subjects: 임학
• ISSN: 2288-9744; 2288-9752

Iris koreana (Iridaceae) is an endangered plant native to Korea. In order to develop an in vitro propagation method, we investigated the effect of 2,4-dichlorophenoxy acetic acid (2,4-D) and a-naphthalene acetic acid (NAA) on callus induction in different I. koreana tissues. In addition, we also investigated the effect of 2,4-D and Benzyl aminopurine (BA) treatments on adventitious shoot induction in viable calli and the effect of indole-3-butyric acid (IBA) on root formation in viable shoots. We found that callus production was highest with 1.0 mg/L NAA (94.4% cultured rhizome explants), and adding low concentrations of 2,4-D to BA containing media significantly increased the frequency of shoot primordial formation. The best rooting results were obtained with 1.0 mg/L IBA, on which 98% of regenerated shoots developed roots and produced an average of 7.4 roots within 45 days. This in vitro propagation protocol will be useful for conservation, as well as for mass propagation. KCI Citation Count: 0