Multiplex polymerase chain reaction tests for detection of pathogens associated with gastroenteritis

A wide range of enteric pathogens can cause infectious gastroenteritis. Conventional diagnostic algorithms are time-consuming and often lack sensitivity and specificity. Advances in molecular technology have provided new clinical diagnostic tools. Multiplex polymerase chain reaction (PCR)-based test...

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Bibliographic Details
Published inClinics in laboratory medicine Vol. 35; no. 2; p. 461
Main Authors Zhang, Hongwei, Morrison, Scott, Tang, Yi-Wei
Format Journal Article
LanguageEnglish
Published United States 01.06.2015
Subjects
Diagnosis, Differential
Feces - microbiology
Feces - parasitology
Feces - virology
Gastroenteritis - diagnosis
Gastroenteritis - microbiology
Gastroenteritis - parasitology
Gastroenteritis - virology
Humans
Multiplex Polymerase Chain Reaction - methods
Sensitivity and Specificity
Multiplex PCR
Gastroenteritis
Laboratory-developed test
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Summary:A wide range of enteric pathogens can cause infectious gastroenteritis. Conventional diagnostic algorithms are time-consuming and often lack sensitivity and specificity. Advances in molecular technology have provided new clinical diagnostic tools. Multiplex polymerase chain reaction (PCR)-based testing has been used in gastroenterology diagnostics in recent years. This article presents a review of recent laboratory-developed multiplex PCR tests and current commercial multiplex gastrointestinal pathogen tests. It focuses on two commercial syndromic multiplex tests: Luminex xTAG Gastrointestinal Pathogen Panel and BioFire FilmArray gastrointestinal test. Multiplex PCR tests have shown superior sensitivity to conventional methods for detection of most pathogens.
ISSN:1557-9832
DOI:10.1016/j.cll.2015.02.006