Effect of egg yolk antibody on experimental Cryptosporidium parvum infection in scid mice

• Published in: Vaccine Vol. 23; no. 2; pp. 232 - 235
• Main Authors: Kobayashi, Chizu, Yokoyama, Hideaki, Nguyen, Sa Van, Kodama, Yoshikatsu, Kimata, Tsutomu, Izeki, Motohiro
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 25.11.2004; Elsevier
• Subjects: Animals; Antibodies, Protozoan - immunology; Antibodies, Protozoan - therapeutic use; Applied microbiology; Biological and medical sciences; Cryptosporidiosis; Cryptosporidiosis - pathology; Cryptosporidiosis - therapy; Cryptosporidium parvum; Cryptosporidium parvum - immunology; Cryptosporidium parvum - ultrastructure; Egg Yolk; Fundamental and applied biological sciences. Psychology; Human protozoal diseases; IgY; Immunotherapy, Adoptive; Infectious diseases; Medical sciences; Mice; Mice, SCID; Microbiology; Parasitic diseases; Passive immunization; Protozoal diseases; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects); Cryptosporidium parvum; Passive immunization; IgY; Protozoa; Apicomplexa; Protozoal disease; Immunization; Antibody; Rodentia; Vaccine; Parasitosis; Infection; Cryptosporidiosis; Vertebrata; Mammalia; Mouse; Severe; Combined immune deficiency
• ISSN: 0264-410X; 1873-2518
• DOI: 10.1016/j.vaccine.2004.05.034

In this study the effect of chicken egg yolk antibody (IgY) against Cryptosporidium parvum infection was examined. IgY sample was prepared from eggs of chickens immunized with C. parvum oocyst antigens. In vitro, antibody-treated sporozoites showed reduced binding to Caco-2 cells and lost vitality. These phenomena were not observed with a control IgY sample prepared from eggs of non-immunized chickens. Scid mice orally administered with the antibody demonstrated partial reduction in oocyst shedding after challenge with 10 3 oocysts. IgY, however, could not eliminate the infection after 17 days of continuous treatment. The potentials of using specific IgY for treatment and prevention of cryptosporidiosis were discussed.