Halogenated furanones from the red alga, Delisea pulchra, inhibit carbapenem antibiotic synthesis and exoenzyme virulence factor production in the phytopathogen Erwinia carotovora

The plant pathogen Erwinia carotovora regulates expression of virulence factors and antibiotic production via an N-3-oxohexanoyl- L-homoserine lactone (3-oxo-C6-HSL) dependent quorum sensing mechanism. The marine alga Delisea pulchra produces halogenated furanones known to antagonise 3-oxo-C6-HSL ac...

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Bibliographic Details
Published inFEMS microbiology letters Vol. 205; no. 1; pp. 131 - 138
Main Authors Manefield, Michael, Welch, Martin, Givskov, Michael, Salmond, George P.C., Kjelleberg, Staffan
Format Journal Article
LanguageEnglish
Published Elsevier B.V 27.11.2001
Subjects
Carbapenem
Delisea pulchra
Erwinia carotovora
Halogenated furanone
Phytopathogen
Quorum sensing
Erwinia carotovora
Carbapenem
Quorum sensing
Phytopathogen
Delisea pulchra
Halogenated furanone
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Summary:The plant pathogen Erwinia carotovora regulates expression of virulence factors and antibiotic production via an N-3-oxohexanoyl- L-homoserine lactone (3-oxo-C6-HSL) dependent quorum sensing mechanism. The marine alga Delisea pulchra produces halogenated furanones known to antagonise 3-oxo-C6-HSL activity. We have tested the effects of a halogenated furanone on the production of carbapenem, cellulase and protease in E. carotovora. Despite differences in the regulatory mechanisms controlling carbapenem and exoenzyme production each was inhibited by the algal metabolite. We present evidence to suggest that the furanone dependent inhibition of carbapenem production is a result of the disruption of the 3-oxo-C6-HSL dependent expression of the carABCDEFGH operon.
ISSN:0378-1097
1574-6968
DOI:10.1016/S0378-1097(01)00460-8