F-actin contractile rings in protoplasts of the yeast Schizosaccharomyces

• Published in: Cell biology international reports Vol. 15; no. 7; pp. 607 - 610
• Main Authors: JOCHOVA, J, RUPES, I, STREIBLOVA, E
• Format: Journal Article
• Language: English
• Published: London Elsevier B.V 01.07.1991; San Diego, CA Academic Press; New York, NY
• Subjects: Actins - metabolism; Analytical, structural and metabolic biochemistry; Biological and medical sciences; Cell Wall - metabolism; Contractile proteins; Cytoskeleton - metabolism; Fundamental and applied biological sciences. Psychology; Holoproteins; Proteins; Protoplasts - metabolism; Schizosaccharomyces - metabolism; Species Specificity; Schizosaccharomyces japonicus; Yeast; Contractile protein; Actins; Microfilament; Cell wall; Fungi; Protoplast; Microorganism culture; Schizosaccharomyces pombe; Cytoskeleton; Ascomycetes; Thallophyta
• ISSN: 0309-1651; 1878-240X
• DOI: 10.1016/0309-1651(91)90007-6

By rhodamine-phalloidin fluorescence, distinct continuous F-actin rings were visualized in 18 – 20% of the protoplasts of Schizosaccharomyces pombe and S. japonicus var. versatilis, in addition to randomly distributed F-actin dots. Whereas the reversion of ring-lacking protoplasts coincided with the polarization of the dotted F-actin pattern, the ring-containing protoplasts became furrowed as the F-actin rings constricted. The furrowing was more conspicuous in S. japonicus var. versatilis than in S. pombe protoplasts and it was blocked when the reversion was inhibited by Novozyme 234 indicating that the cell wall formation is essential for the F-actin ring constriction.