Disassembly of focal adhesions during apoptosis of endothelial cell line ECV304 infected with Orientia tsutsugamushi

Many bacterial pathogens induce apoptosis in their host cells. We observed the cellular effect of ECV304 cells infected with Orientia tsutsugamushi. The infected cells became rounded and floated in culture supernatant. These floating cells as well as adherent cells exhibited typical features of apop...

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Bibliographic Details
Published inMicrobial pathogenesis Vol. 27; no. 5; pp. 265 - 271
Main Authors Kee, Sun-Ho, Cho, Kyung-A, Kim, Mee-Kyung, Lim, Byung-Uk, Chang, Woo-Hyun, Kang, Jae-Seung
Format Journal Article
LanguageEnglish
Published Oxford Elsevier Ltd 01.11.1999
Elsevier
Subjects
Apoptosis - physiology
Bacteriology
Biological and medical sciences
Cell Adhesion
Cell Line
ECV304 cell line
Endothelium, Vascular - cytology
Endothelium, Vascular - microbiology
Fluorescent Antibody Technique, Indirect
focal adhesions
Fundamental and applied biological sciences. Psychology
Humans
Immunoblotting
In Situ Nick-End Labeling
Microbiology
Orientia tsutsugamushi
Orientia tsutsugamushi - growth & development
Orientia tsutsugamushi - pathogenicity
Orientia tsutsugamushi, apoptosis, focal adhesions
Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains
Orientia tsutsugamushi, apoptosis, focal adhesions
Human
Endothelial cell
Host agent relation
Adhesion
In vitro
Pathogenicity
Rickettsiales
Cell line
Rickettsia tsutsugamushi
Cell death
Bacteria
Cytopathology
Extracellular matrix
Rickettsieae
Rickettsiaceae
Apoptosis
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Summary:Many bacterial pathogens induce apoptosis in their host cells. We observed the cellular effect of ECV304 cells infected with Orientia tsutsugamushi. The infected cells became rounded and floated in culture supernatant. These floating cells as well as adherent cells exhibited typical features of apoptosis, such as DNA fragmentation and TUNEL staining. As many cells detached from growth substrate, we examined the focal adhesion using the immunofluorescence assay method and observed decreased focal adhesions in heavily infected cells. As endothelial cells could undergo apoptosis by the loss of focal adhesions, this change of focal adhesions may account for the Orientia -induced apoptosis.
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ISSN:0882-4010
1096-1208
DOI:10.1006/mpat.1999.0304