Aspergillus niger G proteins: subcellular localization

Aspergillus niger postmitochondrial fraction, which contains high GTPase activity and high GTP binding capacity, has been subjected to subcellular fractionation on a sucrose gradient. A cytosolic and four membranous populations have been separated according to their relative density. The main diffic...

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Bibliographic Details
Published inFEMS microbiology letters Vol. 71; no. 3; p. 259
Main Authors Mérit, X, Frot-Coutaz, J, Got, R, Létoublon, R
Format Journal Article
LanguageEnglish
Published England 01.05.1992
Subjects
Aspergillus niger - chemistry
Cell Membrane - chemistry
Cytosol - chemistry
Endoplasmic Reticulum - chemistry
GTP-Binding Proteins - isolation & purification
Subcellular Fractions - chemistry
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Summary:Aspergillus niger postmitochondrial fraction, which contains high GTPase activity and high GTP binding capacity, has been subjected to subcellular fractionation on a sucrose gradient. A cytosolic and four membranous populations have been separated according to their relative density. The main difficulty has been the characterization of the plasma membrane of the fungus. This fraction, which does not contain any typical enzyme, has been identified after iodination of the outer proteins of protoplasts from A. niger. The immunological detection has shown the occurrence of cytosolic G proteins and membranous small G proteins located not only in the plasma membrane but also in the membranes of the endoplasmic reticulum.
ISSN:0378-1097
DOI:10.1016/0378-1097(92)90719-5