Use of an automatic DNA sequencer for S1 mapping: transcriptional analysis of the Streptomyces coelicolor A3(2) dnaK operon

The transcription start point of the dnaK operon of Streptomyces coelicolor A3(2) has been determined by S1 mapping, using the EMBL automated fluorescent DNA sequencer. The −35 and −10 hexamers correspond to a σ 70-type promoter. This promoter responds to heat shock and involves an inverted repeat d...

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Bibliographic Details
Published inFEMS microbiology letters Vol. 149; no. 2; pp. 189 - 194
Main Authors Brans, Alain, Loriaux, Axelle, Thamm, Iris, Joris, Bernard, Dusart, Jean
Format Journal Article Web Resource
LanguageEnglish
Published Elsevier B.V 15.04.1997
Oxford University Press
Subjects
Biochemistry, biophysics & molecular biology
Biochimie, biophysique & biologie moléculaire
Chaperone
CIRCE motif
dnaK operon
Heat shock promoter
Heat shock protein
Life sciences
Sciences du vivant
Heat shock promoter
dnaK operon
Chaperone
CIRCE motif
Heat shock protein
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Summary:The transcription start point of the dnaK operon of Streptomyces coelicolor A3(2) has been determined by S1 mapping, using the EMBL automated fluorescent DNA sequencer. The −35 and −10 hexamers correspond to a σ 70-type promoter. This promoter responds to heat shock and involves an inverted repeat different from the CIRCE sequence characteristic of the Gram-positive heat-shock promoters.
Bibliography:scopus-id:2-s2.0-0030947112
ISSN:0378-1097
1574-6968
1574-6968
DOI:10.1016/S0378-1097(97)00074-8