Separation and determination of the anthraquinones in Xanthophytum attopvensis pierre by nonaqueous capillary electrophoresis

• Published in: Talanta (Oxford) Vol. 65; no. 1; pp. 15 - 20
• Main Authors: Li, Yuqin, Qi, Shengda, Chen, Xingguo, Hu, Zhide
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 15.01.2005; Oxford Elsevier
• Subjects: Analytical chemistry; Anthraquinones; Chemistry; Chromatographic methods and physical methods associated with chromatography; Exact sciences and technology; Nonaqueous capillary electrophoresis; Other chromatographic methods; Xanthophytum attopvensis pierre; Xanthophytum attopvensis pierre; Anthraquinones; Nonaqueous capillary electrophoresis; Capillary electrophoresis; Glucoside; Direct method; Plant; Anthraquinone; Ultraviolet detector; Sample preparation; Sodium; Dicotyledones; Angiospermae; Acetonitrile; Rubiaceae; Spermatophyta; Acetic acid; Methanol
• ISSN: 0039-9140; 1873-3573
• DOI: 10.1016/j.talanta.2004.04.019

A nonaqueous capillary electrophoresis (NACE) method with direct on-column UV detection has been developed for the separation of the pharmaceutically important anthraquinones from the total grass of Xanthophytum attopvensis pierre extract. The separation of three main anthraquinones (1-hydroxy-2-methoxy-3-hydroxymethyl-9, 10-anthraquinone-1- O-β- d-glucoside ( 1), rubiadin- 1-methylether ( 2) and 1-methoxy-2-formyl-3-hydroxy-9, 10-anthraquinone ( 3)) was optimized with respect to concentration of sodium cholate (SC) and acetic acid, addition of acetonitrile (ACN), and applied voltage. Baseline separation was obtained for the three analytes within 5 min using a running buffer containing 50 mM sodium cholate (SC), 1.0% acetic acid and 40% ACN in methanol. The method of NACE for the separation and determination of bioactive ingredient in traditional Chinese medicines was discussed.