Isolation of Alcohol-tolerant Amylolytic Saccharomyces cerevisiae and Its Application to Alcohol Fermentation

An novel amylolytic yeast, Saccharomyces cerevisiae HA 27, isolated from nuruk, displayed resistance against high sugar (50% glucose) and alcohol (15%). Maximal production of amylolytic enzyme by S. cerevisiae HA 27 was achieved oil 9 days of cultivation at the optimal temperature 20℃ and pH 6.0. Th...

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Published inFood science and biotechnology Vol. 17; no. 6
Main Authors Jung, H.K. (Bio Industry Center, Daegu Technopark, Daegu, Republic of Korea), Park, C.D. (Bio Industry Center, Daegu Technopark, Daegu, Republic of Korea), Bae, D.H. (Konkuk University, Seoul, Republic of Korea), Hong, J.H. (Bio Industry Center, Daegu Technopark, Daegu, Republic of Korea), E-mail: betabio@empal.com
Format Journal Article
LanguageEnglish
Published 01.12.2008
Subjects
alcohol-tolerance
amylolytic yeast
nuruk
SACCHAROMYCES CEREVISIAE
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Summary:An novel amylolytic yeast, Saccharomyces cerevisiae HA 27, isolated from nuruk, displayed resistance against high sugar (50% glucose) and alcohol (15%). Maximal production of amylolytic enzyme by S. cerevisiae HA 27 was achieved oil 9 days of cultivation at the optimal temperature 20℃ and pH 6.0. The activity of amylolytic enzyme produced by S. cerevisiae HA 27 was stable, even at 70℃, and over a broad pH range (4.0-11.0). Also, the amylolytic enzyme of S. cerevisiae HA 27 showed optimal activity in pH 5.0 at 50℃. S. cerevisiae HA 27 exhibited 6.2%(v/v) alcohol fermentation ability using starch as a carbon source.
Bibliography:2009001048
Q01
ISSN:1226-7708
2092-6456