Column selectivity in reversed-phase liquid chromatography: II. Effect of a change in conditions

• Published in: Journal of Chromatography A Vol. 961; no. 2; pp. 195 - 215
• Main Authors: Wilson, N.S., Nelson, M.D., Dolan, J.W., Snyder, L.R., Carr, P.W.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 05.07.2002; Elsevier
• Subjects: Analysis; Analytical chemistry; Biological and medical sciences; Buffers; Chemistry; Chromatographic methods and physical methods associated with chromatography; Chromatography, Liquid - instrumentation; Exact sciences and technology; General pharmacology; Hydrogen-Ion Concentration; Medical sciences; Other chromatographic methods; Pharmacology. Drug treatments; Sensitivity and Specificity; Silanes - chemistry; Retention prediction; Selectivity; Temperature effects; Stationary phase, LC; Mobile phase composition; Column selectivity; Drug; Elution; Gradient; Phase composition; Property composition relationship; Chromatographic retention; Ion pair; Prediction; HPLC chromatography; Temperature effect; Theoretical study; Regression analysis; Experimental study; Mobile phase; Isocratic condition; Organic acids; Reversed phase chromatography; Basic compound; Correlation analysis; Neutral compound; pH; Property structure relationship; Bonded stationary phase
• ISSN: 0021-9673
• DOI: 10.1016/S0021-9673(02)00660-X

The isocratic retention of 67 widely-different solutes in reversed-phase liquid chromatography (RP-LC) has been investigated as a function of temperature and mobile phase composition (% B) for three different C 18 columns. Similar studies were also carried out in a gradient mode, where temperature, gradient time and solvent type were varied. These results show that changes in retention with these conditions are similar for each of these three columns. This suggests that relative column selectivity as defined by experiments for one set of experimental conditions will be approximately applicable for other conditions, with the exception of changes in mobile phase pH—which can affect values of the column parameter C (a measure of silanol ionization). Column selectivity as a function of pH was explored for several columns.