Intranasal murine model of Bordetella pertussis infection: II. Sequence variation and protection induced by a tricomponent acellular vaccine

• Published in: Vaccine Vol. 17; no. 20; pp. 2651 - 2660
• Main Authors: Boursaux-Eude, Caroline, Thiberge, Sabine, Carletti, Georges, Guiso, Nicole
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 04.06.1999; Elsevier
• Subjects: Acellular vaccine; Adhesins, Bacterial - genetics; Amino Acid Sequence; Animals; Bacterial Outer Membrane Proteins - genetics; Bacteriology; Biological and medical sciences; Bordetella pertussis; Bordetella pertussis - classification; Diphtheria-Tetanus-Pertussis Vaccine - immunology; Female; Fundamental and applied biological sciences. Psychology; Genes, Bacterial; Hemagglutinins - genetics; Lung - microbiology; Mice; Mice, Inbred BALB C; Microbiology; Molecular Sequence Data; Murine model of protection; Pertussis Toxin; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies; Vaccines, Synthetic - immunology; Virulence Factors, Bordetella - genetics; Whooping Cough - prevention & control; Acellular vaccine; Murine model of protection; Bordetella pertussis; Immunoprotection; Nucleotide sequence; Rodentia; Polyvalent vaccine; Whooping cough; Infection; Toxin; Vertebrata; Mammalia; Mouse; Pertactin; Bacteriosis; Bacteria; Aminoacid sequence
• ISSN: 0264-410X; 1873-2518
• DOI: 10.1016/S0264-410X(99)00038-9

When pertussis toxin S1 subunit and pertactin structural genes in Bordetella pertussis clinical isolates from France and Germany were sequenced, 3 previously described S1 subunit types (S1 A, B and E), and 4 pertactin types (PRN A, B, C, A*) were found. PRN A*, present in the WHO reference strain 18323, was not described previously. In a respiratory mouse model, a tricomponent acellular pertussis vaccine (Infanrix™) was highly effective in promoting lung clearance of all isolates expressing different S1 subunit and pertactin suggesting that use of acellular vaccine will not increase the risks of pertussis infection by these B. pertussis variants.