Characterization of slow postsynaptic K + current of Aplysia LUQ neurons in culture
We have characterized the slow inhibitory synaptic connection between an left upper quadrant (LUQ) neuron and neuron L10, L12 or L13 in culture. A slow postsynaptic outward current in the LUQ neuron was elicited by repetitive firing of the co-cultured cholinergic neuron L10 as well as neuron L12 or...
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Published in | Brain research Vol. 629; no. 1; pp. 88 - 94 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
London
Elsevier B.V
26.11.1993
Amsterdam Elsevier New York, NY |
Subjects | |
Online Access | Get full text |
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Summary: | We have characterized the slow inhibitory synaptic connection between an left upper quadrant (LUQ) neuron and neuron L10, L12 or L13 in culture. A slow postsynaptic outward current in the LUQ neuron was elicited by repetitive firing of the co-cultured cholinergic neuron L10 as well as neuron L12 or L13 which were not cholinergic but FMRFamide synthesizing neurons. This outward current was due to an increase in K
+ conductance and was relatively insensitive to external application of tetraethylammonium and 4-aminopyridine. We also investigated the effects of injection of a guanosine 5′-triphosphate (GTP) analogue into the LUQ neuron and extracellular application of serotonin and an adenosine 3′,5′-cyclic monophosphate (cAMP) analogue on the current. In all the investigated properties, no significant difference was found among neuron L10, L12 and L13 as a presynaptic neuron. The slow postsynaptic current appeared to be virtually identical to the FMRFamide or acetylcholine (ACh)-induced K
+ current reported in vivo, which resembled the ‘S’ current in
Aplysia sensory neurons. We performed experiments to see the effect of phenyltrimethylamminium, an ACh antagonist, on the postsynaptic, ACh-, and FMRFamide-induced currents, clearly indicating that the neurotransmitter used in the cultured synapses was not ACh. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0006-8993 1872-6240 |
DOI: | 10.1016/0006-8993(93)90485-6 |