Neuroprotective potential of pyrazole benzenesulfonamide derivative T1 in targeted intervention against PTZ-induced epilepsy-like condition in in vivo zebrafish model
[Display omitted] •Pyrazole benzenesulfonamide derivative T1 inhibits COX-2 targeted intervention.•T1 shields against PTZ-induced cellular damage, affirming neuroprotective effects.•The convulsive response in PTZ-exposed zebrafish was ameliorated by T1 treatment.•Gene expression and neutral red assa...
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Published in | International immunopharmacology Vol. 131; p. 111859 |
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Main Authors | , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Netherlands
Elsevier B.V
20.04.2024
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Subjects | |
Online Access | Get full text |
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Summary: | [Display omitted]
•Pyrazole benzenesulfonamide derivative T1 inhibits COX-2 targeted intervention.•T1 shields against PTZ-induced cellular damage, affirming neuroprotective effects.•The convulsive response in PTZ-exposed zebrafish was ameliorated by T1 treatment.•Gene expression and neutral red assay highlight T1 reduces inflammatory response.
Epilepsy is a chronic neurological disease characterized by a persistent susceptibility to seizures. Pharmaco-resistant epilepsies, impacting around 30 % of patients, highlight the urgent need for improved treatments. Neuroinflammation, prevalent in epileptogenic brain regions, is a key player in epilepsy, prompting the search for new mechanistic therapies. Hence, in this study, we explored the anti-inflammatory potential of pyrazole benzenesulfonamide derivative (T1) against pentylenetetrazole (PTZ) induced epilepsy-like conditions in in-vivo zebrafish model. The results from the survival assay showed 79.97 ± 6.65 % at 150 µM of T1 compared to PTZ-group. The results from reactive oxygen species (ROS), apoptosis and histology analysis showed that T1 significantly reduces cellular damage due to oxidative stress in PTZ-exposed zebrafish. The gene expression analysis and neutral red assay results demonstrated a notable reduction in the inflammatory response in zebrafish pre-treated with T1. Subsequently, the open field test unveiled the anti-convulsant activity of T1, particularly at a concentration of 150 μM. Moreover, both RT-PCR and immunohistochemistry findings indicated a concentration-dependent potential of T1, which inhibited COX-2 in zebrafish exposed to PTZ. In summary, T1 protected zebrafish against PTZ-induced neuronal damage, and behavioural changes by mitigating the inflammatory response through the inhibition of COX-2. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1567-5769 1878-1705 |
DOI: | 10.1016/j.intimp.2024.111859 |