MiR-370 functions as oncogene in melanoma by direct targeting pyruvate dehydrogenase B

• Published in: Biomedicine & pharmacotherapy Vol. 90; pp. 278 - 286
• Main Authors: Wei, Shufang, Ma, Weiyuan
• Format: Journal Article
• Language: English
• Published: France 01.06.2017
• Subjects: Apoptosis - genetics; Cell Line, Tumor; Cell Movement - genetics; Cell Proliferation - genetics; Down-Regulation - genetics; Gene Expression Regulation, Neoplastic - genetics; Glycolysis - genetics; Humans; Internal Medicine; Medical Education; Melanoma - genetics; MicroRNAs - genetics; Neoplasm Invasiveness - genetics; Oncogenes - genetics; Oxidoreductases - metabolism; Pyruvates - metabolism; Up-Regulation - genetics; MiR-370; Melanoma; PDHB
• ISSN: 0753-3322; 1950-6007; 1950-6007
• DOI: 10.1016/j.biopha.2017.03.068

Abstract Background Accumulating evidence has shown that miR-370 play an important role in the development and progression of tumor. However, the role of miR-370 in melanoma remains largely unknown. The present study is designed to investigate the function of miR-370 in melanoma and to explore the molecular mechanism underlying its function. Materials and methods The expression level of miR-370 was detected in melanoma tissues and cell lines by real-time quantitative polymerase chain reaction (qRT-PCR). The effect of overexpression of miR-370 on in vitro cell proliferation, apoptosis, invasion as well as glyclolysis was examined. Western blotting analysis was used to detect the influence of miR-370 on the expression of target genes, and Pearson analysis was used to calculate the correlation between the expression of targets gene and miR-370 in melanoma tissues. Results Our study showed that miR-370 was upregulated in melanoma tissues compared with non-cancerous tissues (P < 0.01). In addition, the expression of miR-370 in melanoma cell lines was also significantly higher (P < 0.01). Enforced expression of miR-370 promotes melanoma cell proliferation, inhibits apoptosis and enhances invasion and glycolysis and led to downregulation of the PDHB protein. Moreover, the expression level of miR-370 in melanoma tissues showed inverse relationship with the expression level of PDHB protein. Conclusions In conclusion, our findings suggested that miR-370 represents a potential oncogenic miRNA and plays an important role in melanoma progression by directly targeting PDHB.