Cyclic GMP production and excretion by Drosophila cells : Modulation by 20-hydroxyecdysone

Intracellular and extracellular concentrations of cyclic GMP were measured under different conditions in cultures of two 20-hydroxyecdysone sensitive clones of Drosophila melanogaster. Exponentially growing cells maintained a concentration of 0.15 pmol/10 6 cells inside the cells (about 10 −6 M) and...

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Bibliographic Details
Published inInsect biochemistry Vol. 14; no. 6; pp. 691 - 695
Main Authors Courgeon, Anne-Marie, Cailla, Hélène L.
Format Journal Article
LanguageEnglish
Published Oxford Elsevier B.V 1984
New York, NY Pergamon Press
Subjects
Biochemistry. Physiology. Immunology
Biological and medical sciences
Drosophila melanogaster
ecdysteroids
excretion
Fundamental and applied biological sciences. Psychology
guanosine monophosphate
Insecta
Invertebrates
Physiology. Development
excretion
Drosophila melanogaster
Cyclic GMP
20-hydroxyecdysone
Ecdysteroide
Steroid hormone
Arthropoda
Insecta
Cyclic nucleotide
3',5'-GMP
Invertebrata
Metabolism
Diptera
Ecdysone β
Drosophilidae
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Summary:Intracellular and extracellular concentrations of cyclic GMP were measured under different conditions in cultures of two 20-hydroxyecdysone sensitive clones of Drosophila melanogaster. Exponentially growing cells maintained a concentration of 0.15 pmol/10 6 cells inside the cells (about 10 −6 M) and 5 pmol/10 6 cells in the medium. When the medium was changed equilibrium was restored in the cells in about 15 hr. Addition of 20-hydroxyecdysone arrested growth and induced several enzymes and morphological modifications. It also increased (up to 10-fold) both intracellular and extracellular cyclic GMP levels depending on the amount of hormone. The ratio of the two levels remained constant.
ISSN:0020-1790
DOI:10.1016/0020-1790(84)90048-9