Human mammary slices in organ culture: I. Method of culture and preliminary observations on the effect of insulin

A technique for culture of human mammary slices has been devised, which permits survival and limited proliferation of all elements for periods up to 12 days in vitro. Slices survive in a variety of media with or without serum, providing that glucose concentrations are maintained above critical level...

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Bibliographic Details
Published inExperimental cell research Vol. 35; no. 3; pp. 437 - 448
Main Authors Barker, B.E., Fanger, H., Farnes, P.
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 01.01.1964
Subjects
Animals
Breast
Cell Division
Epithelium
Histology
Histology, Comparative
Humans
Hypertrophy
Insulin
Mammary Glands, Animal
Metaplasia
Mice
Old Medline
Organ Culture Techniques
Pharmacology
Tissue Culture Techniques
CELL DIVISION
MAMMAE
EXPERIMENTAL LAB STUDY
HISTOLOGY
TISSUE CULTURE
BREAST
INSULIN
HYPERTROPHY
EPITHELIUM
PHARMACOLOGY
HISTOLOGY, COMPARATIVE
METAPLASIA
MICE
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Summary:A technique for culture of human mammary slices has been devised, which permits survival and limited proliferation of all elements for periods up to 12 days in vitro. Slices survive in a variety of media with or without serum, providing that glucose concentrations are maintained above critical levels. In these organ cultures, addition of insulin to the medium results in marked morphological changes of ductal epithelium including proliferation, hypertrophy, and foci resembling squamous metaplasia.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0014-4827
1090-2422
DOI:10.1016/0014-4827(64)90134-X