Cytotoxic and apoptotic activities of Tussilago farfara extract in HT-29 human colon cancer cells

The flower buds of Tussilago farfara (TF) have been traditionally used in oriental medicine for the treatment of bronchitis and asthma. In our study, the primary objective was to determine the mechanisms that are inherent to TF-induced cytotoxicity and apoptosis, using the methanolic extract of TF (...

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Published inFood science and biotechnology Vol. 17; no. 2
Main Authors Lee, M.R. (Kyungnam University, Masan, Republic of Korea), Cha, M.R. (Kyungnam University, Masan, Republic of Korea), Jo, K.J. (Kyungnam University, Masan, Republic of Korea), Yoon, M.Y. (Kyungnam University, Masan, Republic of Korea), Park, H.R. (Kyungnam University, Masan, Republic of Korea), E-mail: parkhy@kyungnam.ac.kr
Format Journal Article
LanguageEnglish
Published 01.04.2008
Subjects
APOPTOSE
APOPTOSIS
HT-29 cell
oriental medicine
TOXICIDAD
TOXICITE
TOXICITY
Tussilago farfara
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Summary:The flower buds of Tussilago farfara (TF) have been traditionally used in oriental medicine for the treatment of bronchitis and asthma. In our study, the primary objective was to determine the mechanisms that are inherent to TF-induced cytotoxicity and apoptosis, using the methanolic extract of TF (TFM) in HT-29 human colon cancer cells. We found that TFM-induced induced cytotoxicity in HT-29 cells in a dose-dependent manner. This effect was verified via an MTT reduction assay, an lactate dehydrogenase (LDH) release assay, and a colony formation assay. Interestingly, we also detected apoptotic bodies on Hoechst staining, and attempted to determine whether TFNI-induced apoptosis involved the caspase pathway using a caspase-3/7 activity assay. Overall, the results indicate that TFM contain chemotherapeutic agents and potential candidates use for against human colon cancer cells.
Bibliography:Q01
2008003827
ISSN:1226-7708
2092-6456