Effects of Aucubin Isolated from Eucommia ulmoides on UVB-induced Oxidative Stress in Human Keratinocytes HaCaT

• Published in: Food science and biotechnology Vol. 18; no. 2
• Main Authors: Ho, J.N., Han-Seo University, Seosan, Republic of Korea, Cho, H.Y., Korea University, Seoul, Republic of Korea, Lim, E.J., Hanyang Women's College, Seoul, Republic of Korea, Kim, H.K., Han-Seo University, Seosan, Republic of Korea
• Format: Journal Article
• Language: English
• Published: 01.04.2009
• Subjects: aucubin; ESTRES OXIDATIVO; matrix metalloproteinase-1 (MMP-1); OXIDATIVE STRESS; photoaging; STRESS OXYDATIF; ultraviolet B (UVB)
• ISSN: 1226-7708

Ultraviolet B (UVB) radiation provokes the generation of reactive oxygen species (ROS) in the cells and skin, which induce oxidative stress in the exposed cells, leading to photoaging and cancer. Using the human keratinocytes HaCaT cell line, we investigated the photoprotective effects of aucubin isolated from Eucommia ulmoides. Pretreatment with aucubin markedly suppressed UVB-induced oxidative stress, which manifests as a decrease in intracellular lipid peroxidation, elevation of catalase activity, and reduced glutathione content. In addition, aucubin significantly reduced expression of matrix metalloproteinase-1 (MMP-1) protein (54%) and mRNA. Taken together, these results suggest that aucubin may offer protection against UVB-induced oxidative stress and may be used as a potential agent in prevention of UVB-induced photoaging.