Properties of Scleroderma Fibroblasts in Culture

Skin fibroblasts from normal control subjects and subjects with scleroderma were compared for growth characteristics in culture. Optimal growth for the scleroderma fibroblasts, unlike that for controls, was observed to be dependent on elevated levels of fetal calf serum. Culturing scleroderma fibrob...

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Bibliographic Details
Published inJournal of investigative dermatology Vol. 63; no. 6; pp. 456 - 460
Main Authors Fleishmajer, Raul, Kovacs, Charles J.
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 01.12.1974
Subjects
Animals
Cattle
Cell Division
Cell Separation
Cells, Cultured
Culture Media
Fetus
Fibroblasts - pathology
Humans
Scleroderma, Systemic - pathology
Time Factors
Trypsin - pharmacology
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Summary:Skin fibroblasts from normal control subjects and subjects with scleroderma were compared for growth characteristics in culture. Optimal growth for the scleroderma fibroblasts, unlike that for controls, was observed to be dependent on elevated levels of fetal calf serum. Culturing scleroderma fibroblasts in Eagle's minimal essential medium (MEM) supplemented with 10% fetal calf serum resulted in: a) an increased population-doubling time over control, (b) a failure to attain contact -inhibited confluence, and (c) an altered cell morphology. Both elevated levels of serum and Ham's F12 nutrient mixture successfully restored normal control culture growth characteristics to the scleroderma cultures.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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ISSN:0022-202X
1523-1747
DOI:10.1111/1523-1747.ep12680366