Alterations in lipid incorporation in Duchenne muscular dystrophy: Studies of fresh and cultured muscle

• Published in: Journal of the neurological sciences Vol. 50; no. 2; pp. 249 - 257
• Main Authors: Ionasescu, V., Monaco, L., Sandra, A., Ionasescu, R., Burmeister, L., Deprosse, C., Stern, L.Z.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.01.1981
• Subjects: Adolescent; Adult; Child; Child, Preschool; Culture Techniques; Female; Glycerol - metabolism; Humans; Lipid Metabolism; Male; Muscles - metabolism; Muscular Dystrophies - metabolism; Phosphatidylcholines - metabolism; Phosphatidylinositols - metabolism; Phosphatidylserines - metabolism; Pregnancy; Triglycerides - metabolism
• ISSN: 0022-510X; 1878-5883
• DOI: 10.1016/0022-510X(81)90171-4

The incorporation of [ 3H]glycerol into lipids of fresh and cultured skeletal muscle obtained from patients with Duchenne muscular dystrophy (DMD), patients with myotonic dystrophy (My Dyst), controls, and aborted fetuses (10–12 weeks old) was studied. A significant increase of specific incorporation of [ 3H]glycerol into phosphatidylcholine (PCh), phosphatidylserine (PS), phosphatidylinositol (PI), and triglycerides (TRI) was found in DMD and fetal muscle in both fresh and cultured muscle. No significant differences, however, were noted between the values of glycerol incorporation in DMD and fetal muscle. The ratio between phospholipids and TRI changed significantly for fresh muscle in DMD (3.5) and fetal muscle (4.9) versus controls (24). The incorporation of glycerol into these lipids in My Dyst fresh and cultured muscle showed the same values as controls when expressed both as incorporation/mg protein and ratio between phospholipids and TRI.