Acetylene degradation by new isolates of aerobic bacteria and comparison of acetylene hydratase enzymes
Aerobic acetylene-degrading bacteria were isolated from soil samples. Two isolates were assigned to the species Rhodococcus opacus, two others to Rhodococcus ruber and Gordona sp. They were compared with known strains of aerobic acetylene-, cyanide-, or nitrile-utilizing bacteria. The acetylene hydr...
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| Published in | FEMS microbiology letters Vol. 148; no. 2; pp. 175 - 180 |
|---|---|
| Main Authors | , , , |
| Format | Journal Article |
| Language | English |
| Published |
Elsevier B.V
1997
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| Subjects | |
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| Abstract | Aerobic acetylene-degrading bacteria were isolated from soil samples. Two isolates were assigned to the species
Rhodococcus opacus, two others to
Rhodococcus ruber and
Gordona sp. They were compared with known strains of aerobic acetylene-, cyanide-, or nitrile-utilizing bacteria. The acetylene hydratases of
R. opacus could be measured in cell-free extracts only in the presence of a strong reductant like titanium(III) citrate. Expression of these enzymes was molybdenum-dependent. Acetylene hydratases in cell-free extracts of
R. ruber and
Gordona spp. did not require addition of reductants. No cross-reactivity could be found between cell-free extracts of any of these aerobic isolates and antibodies raised against the acetylene hydratase of the strictly anaerobic fermenting bacterium
Pelobacter acetylenicus. These results show that acetylene hydratases are a biochemically heterogeneous group of enzymes. |
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| AbstractList | Aerobic acetylene-degrading bacteria were isolated from soil samples. Two isolates were assigned to the species
Rhodococcus opacus, two others to
Rhodococcus ruber and
Gordona sp. They were compared with known strains of aerobic acetylene-, cyanide-, or nitrile-utilizing bacteria. The acetylene hydratases of
R. opacus could be measured in cell-free extracts only in the presence of a strong reductant like titanium(III) citrate. Expression of these enzymes was molybdenum-dependent. Acetylene hydratases in cell-free extracts of
R. ruber and
Gordona spp. did not require addition of reductants. No cross-reactivity could be found between cell-free extracts of any of these aerobic isolates and antibodies raised against the acetylene hydratase of the strictly anaerobic fermenting bacterium
Pelobacter acetylenicus. These results show that acetylene hydratases are a biochemically heterogeneous group of enzymes. |
| Author | Kroppenstedt, Reiner M Schink, Bernhard Rosner, Bettina M Rainey, Frederick A |
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| Copyright | 1997 Federation of European Microbiological Societies |
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| Keywords | Gordona Rhodococcus Pelobacter acetylenicus Acetylene degradation Acetylene hydratase |
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(1995) Purification and characterization of acetylene hydratase of sp. nov., an unusual nutritionally versatile Can. J. Microbiol. 34, 242–248. gen. nov., sp. nov. Arch. Microbiol. 129, 395–400. Schygulla-Banek, K. (1993) Verwertung von freiem Cyanid und Eisencyanokomplexen durch ein neuartiges Bakterium. Ph.D. Thesis, University of Stuttgart. species. Microbiology 141, 523–528. |
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| Snippet | Aerobic acetylene-degrading bacteria were isolated from soil samples. Two isolates were assigned to the species
Rhodococcus opacus, two others to
Rhodococcus... |
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| SubjectTerms | Acetylene degradation Acetylene hydratase Gordona Pelobacter acetylenicus Rhodococcus |
| Title | Acetylene degradation by new isolates of aerobic bacteria and comparison of acetylene hydratase enzymes |
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