Acetylene degradation by new isolates of aerobic bacteria and comparison of acetylene hydratase enzymes

Aerobic acetylene-degrading bacteria were isolated from soil samples. Two isolates were assigned to the species Rhodococcus opacus, two others to Rhodococcus ruber and Gordona sp. They were compared with known strains of aerobic acetylene-, cyanide-, or nitrile-utilizing bacteria. The acetylene hydr...

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Bibliographic Details
Published inFEMS microbiology letters Vol. 148; no. 2; pp. 175 - 180
Main Authors Rosner, Bettina M, Rainey, Frederick A, Kroppenstedt, Reiner M, Schink, Bernhard
Format Journal Article
LanguageEnglish
Published Elsevier B.V 1997
Subjects
Acetylene degradation
Acetylene hydratase
Gordona
Pelobacter acetylenicus
Rhodococcus
Gordona
Rhodococcus
Pelobacter acetylenicus
Acetylene degradation
Acetylene hydratase
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Summary:Aerobic acetylene-degrading bacteria were isolated from soil samples. Two isolates were assigned to the species Rhodococcus opacus, two others to Rhodococcus ruber and Gordona sp. They were compared with known strains of aerobic acetylene-, cyanide-, or nitrile-utilizing bacteria. The acetylene hydratases of R. opacus could be measured in cell-free extracts only in the presence of a strong reductant like titanium(III) citrate. Expression of these enzymes was molybdenum-dependent. Acetylene hydratases in cell-free extracts of R. ruber and Gordona spp. did not require addition of reductants. No cross-reactivity could be found between cell-free extracts of any of these aerobic isolates and antibodies raised against the acetylene hydratase of the strictly anaerobic fermenting bacterium Pelobacter acetylenicus. These results show that acetylene hydratases are a biochemically heterogeneous group of enzymes.
ISSN:0378-1097
1574-6968
DOI:10.1016/S0378-1097(97)00030-X