DETECTION OF ANTI-ERYTHROPOIETIN ANTIBODIES IN HAEMODIALYSIS PATIENTS TREATED WITH RECOMBINANT HUMAN-ERYTHROPOIETIN
An enzyme-immunoassay was developed to evaluate the presence of anti-erythropoietin antibodies in plasma samples obtained from renal failure patients treated with recombinant human erythropoietin (rh-EPO). The assay was specific and reproducible. Normal donors had no antibodies to EPO, while 67% of...
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Published in | Pharmacological research Vol. 41; no. 3; pp. 313 - 318 |
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Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
Netherlands
Elsevier Ltd
01.03.2000
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Subjects | |
Online Access | Get full text |
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Summary: | An enzyme-immunoassay was developed to evaluate the presence of anti-erythropoietin antibodies in plasma samples obtained from renal failure patients treated with recombinant human erythropoietin (rh-EPO). The assay was specific and reproducible. Normal donors had no antibodies to EPO, while 67% of treated patients were positive to the assay. While the specificity of anti-EPO IgG antibodies was high, their affinity for the antigen was low. This finding can be explained by the very small differences in the structure of rh-EPO compared to that of natural EPO. The assay described could be useful in evaluating the long-term effects of rh-EPO treatment on the control of anaemia in renal failure patients. 2000 Academic Press@p$hr |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1043-6618 1096-1186 |
DOI: | 10.1006/phrs.1999.0593 |