Incidence of the cblA major subunit pilin gene amongst Burkholderia species

PCR-amplification has been used to screen 75 isolates of the Burkholderia cepacia complex for the cblA pilin gene. PCR-amplified products of the correct size (664 bp) were cloned and sequenced and the sequences compared. Apart from in the control, epidemic cystic fibrosis (CF)-associated B. cepacia...

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Bibliographic Details
Published inFEMS microbiology letters Vol. 196; no. 1; pp. 61 - 66
Main Authors Richardson, J., Stead, D.E., Coutts, R.H.A.
Format Journal Article
LanguageEnglish
Published Elsevier B.V 01.03.2001
Subjects
Bacterial diversity
Burkholderia cepacia
cblA gene sequence
Bacterial diversity
cblA gene sequence
Burkholderia cepacia
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Summary:PCR-amplification has been used to screen 75 isolates of the Burkholderia cepacia complex for the cblA pilin gene. PCR-amplified products of the correct size (664 bp) were cloned and sequenced and the sequences compared. Apart from in the control, epidemic cystic fibrosis (CF)-associated B. cepacia lineage we also identified, for the first time, cblA genes in a unique, non-CF clinical isolate from France and a plant (onion) pathogenic isolate from Italy. The sequence of the cblA gene amplified from the clinical isolate was more diverged from the epidemic lineage than that amplified from the onion pathogenic isolate.
ISSN:0378-1097
1574-6968
DOI:10.1016/S0378-1097(01)00037-4