PQQ-linked extracellular glucose oxidation and chemotaxis towards this cofactor in rhizobia

The presence of the pyrroloquinoline quinone (PQQ)-dependent glucose dehydrogenase in several strains of different Rhizobium species and of Bradyrhizobium japonicum was investigated. It was found that most strains were only able to synthesize the apo-enzyme, whereas in three strains, Rhizobium melil...

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Bibliographic Details
Published inFEMS microbiology letters Vol. 140; no. 2; pp. 179 - 184
Main Authors Boiardi, JoséLuis, Galar, Maria Lina, Neijssel, Oense M.
Format Journal Article
LanguageEnglish
Published Elsevier B.V 01.07.1996
Subjects
Bradyrhizobium japonicum
Chemotaxis
Glucose dehydrogenase
Pyrroloquinoline quinone
Rhizobium spp
Pyrroloquinoline quinone
Glucose dehydrogenase
Chemotaxis
Bradyrhizobium japonicum
Rhizobium spp
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Summary:The presence of the pyrroloquinoline quinone (PQQ)-dependent glucose dehydrogenase in several strains of different Rhizobium species and of Bradyrhizobium japonicum was investigated. It was found that most strains were only able to synthesize the apo-enzyme, whereas in three strains, Rhizobium meliloti 102F34 and Bradyrhizobium japonicum strains USDA110 and USDA138 holo-enzyme activity could be observed. In washed suspensions, however, only Rhizobium meliloti 102F34 produced gluconate from glucose in the absence of added PQQ. All strains showed positive chemotaxis towards PQQ. This chemotaxis could already be observed with nanomolar concentrations of PQQ.
ISSN:0378-1097
1574-6968
DOI:10.1016/0378-1097(96)00176-0