A novel selective medium for the detection of methicillin-resistant Staphylococcus aureus enabling result reporting in under 24 h

A simple selective broth was devised to indicate the presence of methicillin resistant Staphylococcus aureus (MRSA) in clinical samples. The broth comprised nutrient broth supplemented with sodium chloride, ciprofloxacin, colistin and aztreonam as selective agents and also mannitol, trehalose and ph...

Full description

Saved in:
Bibliographic Details
Published inThe Journal of hospital infection Vol. 52; no. 2; pp. 148 - 151
Main Authors Gurran, C, Holliday, M.G, Perry, J.D, Ford, M, Morgan, S, Orr, K.E
Format Journal Article
LanguageEnglish
Published Kent Elsevier Ltd 01.10.2002
Elsevier
Subjects
Antibacterial agents
Antibiotics. Antiinfectious agents. Antiparasitic agents
Bacterial diseases
Bacterial Typing Techniques
Biological and medical sciences
Human bacterial diseases
Humans
Infectious diseases
Medical sciences
Methicillin Resistance
MRSA, selective medium
Pharmacology. Drug treatments
Staphylococcal Infections - diagnosis
Staphylococcal infections, streptococcal infections, pneumococcal infections
Staphylococcus aureus - drug effects
Time Factors
MRSA, selective medium
Enrichment
Performance evaluation
Culture medium
Costs
selective medium
Infection
Sensitivity
Rapid technique
Microbiological investigation
MRSA
Bacteriosis
Microorganism culture
Bacteria
Micrococcales
Isolation
Micrococcaceae
Staphylococcal infection
Staphylococcus aureus
Online AccessGet full text

Cover

More Information
Summary:A simple selective broth was devised to indicate the presence of methicillin resistant Staphylococcus aureus (MRSA) in clinical samples. The broth comprised nutrient broth supplemented with sodium chloride, ciprofloxacin, colistin and aztreonam as selective agents and also mannitol, trehalose and phenol red as an indicator system. In a preliminary study using 228 clinical samples this selective mannitol broth (SMB) proved to be more sensitive than other selective agars for detection of MRSA within 24 h. In an extended study of a further 1124 clinical samples from 470 randomly selected patients, SMB detected 85.1% of MRSA strains present with a specificity of 43.6%. We conclude that SMB offers a convenient, inexpensive and sensitive method for high-throughput screening for MRSA.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ObjectType-Article-1
ObjectType-Feature-2
ISSN:0195-6701
1532-2939
DOI:10.1053/jhin.2002.1260