Exogenous fatty acids and niacin on acute prostaglandin D2 production in human myeloid cells

• Published in: The Journal of nutritional biochemistry Vol. 39; pp. 22 - 31
• Main Authors: Montserrat-de la Paz, Sergio, Bermudez, Beatriz, Lopez, Sergio, Naranjo, Maria C., Romero, Yolanda, Bando-Hidalgo, Maria J., Abia, Rocio, Muriana, Francisco J.G.
• Format: Journal Article
• Language: English
• Published: Elsevier Inc 01.01.2017
• Subjects: Exogenous fatty acids; Myeloid cells; Niacin; Postprandial state; Prostaglandin D2; Postprandial state; Exogenous fatty acids; Myeloid cells; Prostaglandin D2; Niacin
• ISSN: 0955-2863; 1873-4847
• DOI: 10.1016/j.jnutbio.2016.09.007

Niacin activates HCA2 receptor that results in the release of PGD2. However, little is known on PGD2-producing cells and the role of fatty acids. Notably M-CSF macrophages exhibited a timely dependent PGD2 production upon niacin challenge. Short pretreatment of M-CSF macrophages with autologous postprandial TRLs induced the down-regulation of HCA2 gene and up-regulation of genes encoding COX1 and COX2 enzymes in a fatty acid-dependent manner. These effects were paralleled by a higher PGD2 production with postprandial TRL-SFAs. The niacin-mediated transcriptional activity of all genes involved in PGD2 biosynthesis was desensitized in a time-dependent manner by postprandial TRLs, leading to a decreased PGD2 release. In vivo, the net excursions of PGD2 in plasma followed similar fatty acid-dependent patterns as those found for PGD2 release in vitro. The predominant fatty acid class in the diet acutely modulates PGD2 biosynthetic pathway both in vitro and in vivo.