Rapid high performance liquid chromatography method development with high prediction accuracy, using 5 cm long narrow bore columns packed with sub-2 μm particles and Design Space computer modeling

• Published in: Journal of Chromatography A Vol. 1216; no. 45; pp. 7816 - 7823
• Main Authors: Fekete, Szabolcs, Fekete, Jenő, Molnár, Imre, Ganzler, Katalin
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 06.11.2009; Elsevier
• Subjects: Analysis; Analytical chemistry; Biological and medical sciences; Chemistry; Chromatographic methods and physical methods associated with chromatography; Computer-assisted optimization; DryLab; Exact sciences and technology; General pharmacology; Medical sciences; Method development; Other chromatographic methods; Pharmacology. Drug treatments; Sub-2 μm particles; UHPLC; DryLab; UHPLC; Method development; Sub-2 μm particles; Computer-assisted optimization; Antineoplastic agent; Ethinylestradiol; Steroid; Ultra performance liquid chromatography; Chemical analysis; Impurity; Psychotropic; Estrogen; HPLC chromatography; Purity control; Reuptake inhibitor; Mobile phase; Modeling; Optimization; Accuracy; pH; Antidepressant agent; Microparticle; Tablet; Computer aid; Elution; Gradient; Serotonin; Computer simulation; Prediction; Theoretical study; Oral form; Duloxetine; Isocratic condition; Microbore column; Ultraviolet detector; Quality control; Dosage form; Bicalutamide; Contraceptive
• ISSN: 0021-9673
• DOI: 10.1016/j.chroma.2009.09.043

Many different strategies of reversed phase high performance liquid chromatographic (RP-HPLC) method development are used today. This paper describes a strategy for the systematic development of ultrahigh-pressure liquid chromatographic (UHPLC or UPLC) methods using 5 cm × 2.1 mm columns packed with sub-2 μm particles and computer simulation (DryLab ® package). Data for the accuracy of computer modeling in the Design Space under ultrahigh-pressure conditions are reported. An acceptable accuracy for these predictions of the computer models is presented. This work illustrates a method development strategy, focusing on time reduction up to a factor 3–5, compared to the conventional HPLC method development and exhibits parts of the Design Space elaboration as requested by the FDA and ICH Q8R1. Furthermore this paper demonstrates the accuracy of retention time prediction at elevated pressure (enhanced flow-rate) and shows that the computer-assisted simulation can be applied with sufficient precision for UHPLC applications ( p > 400 bar). Examples of fast and effective method development in pharmaceutical analysis, both for gradient and isocratic separations are presented.