Analysis of fatty acids in plasma lipoproteins by gas chromatography–flame ionization detection: Quantitative aspects

• Published in: Analytica chimica acta Vol. 465; no. 1; pp. 337 - 350
• Main Authors: Tvrzická, E, Vecka, M, Staňková, B, Žák, A
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 16.08.2002
• Subjects: Analytical error; Fatty acid; Gas chromatography; Lipoprotein; ECL, equivalent chain length; MS, mass spectrometry; RRT, relative retention time; MHLP, mixed hyperlipoproteinemia; RCF, response correction factor; EDTA, ethylenediaminetetraacetic acid; HDL, high density lipoprotein; HLP, hyperlipoproteinemia; CM, chylomicrons; Lipoprotein; CN, carbon number; PL, phospholipids; RRF, relative response factor; TG, triglycerides; RT, retention time; Fatty acid; TCD, thermal conductivity detector; GC, gas chromatography; IS, internal standard; PUFA, polyunsaturated fatty acids; LDL, low density lipoprotein; LP, lipoprotein; PC, phosphatidylcholine; Analytical error; HC, hypercholesterolemia; PPAR, peroxisome proliferator activated receptor; SFA, saturated fatty acids; FAME, fatty acid methyl esters; MFA, monounsaturated fatty acids; NLP, normolipoproteinemia; RF, response factor; Gas chromatography; CE, cholesteryl esters; FID, flame ionization detector; i.d., internal diameter; VLDL, very low density lipoprotein; PTV, programmed temperature vaporizer; FA, fatty acids; IDL, intermediate density lipoprotein; R.S.D., relative standard deviation; TLC, thin-layer chromatography
• ISSN: 0003-2670; 1873-4324
• DOI: 10.1016/S0003-2670(02)00396-3

Profiles of fatty acids in lipid classes of plasma and ultracentrifugally separated lipoproteins were analyzed by capillary gas chromatography. Contribution of individual steps of the whole procedure was calculated on the basis of the multiple analyses. GC contributes to the overall error by the smallest part. Contribution of the extraction, thin-layer chromatography separation and methylation processes might be comparable. The value of relative standard deviation is indirectly proportional to the amount of analyzed component. Comparison of FA profiles in lipid classes of very low density lipoproteins and low density lipoproteins revealed highly individual differences. Increased content of arachidonic and docosahexaenoic acids in triglycerides of low density lipoproteins are in agreement with formerly published results. Duplicate analyses are recommended for the evaluation of differences between FA composition in individual lipoproteins.