Rapid determination of diazepam and nordiazepam in plasma by electron capture gas—liquid chromatography : Application in clinical pharmacokinetic studies

A rapid method was developed for the determination of diazepam and nordiazepam (N-desmethyldiazepam) in human plasma using electron capture gas—liquid chromatography (GLC—ECD). The concentration of diazepam and nordiazepam is determined using 0.5 ml of plasma extracted with 1.0 ml of benzene contain...

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Bibliographic Details
Published inJournal of chromatography Vol. 143; no. 6; pp. 581 - 595
Main Authors Weinfeld, Robert E., Posmanter, Howard N., Khoo, Ko-Chin, Puglisi, Carl V.
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier B.V 01.11.1977
Subjects
Autoanalysis
Chromatography, Gas
Chromatography, Liquid
Diazepam - administration & dosage
Diazepam - analogs & derivatives
Diazepam - blood
Humans
Nordazepam - administration & dosage
Nordazepam - blood
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Summary:A rapid method was developed for the determination of diazepam and nordiazepam (N-desmethyldiazepam) in human plasma using electron capture gas—liquid chromatography (GLC—ECD). The concentration of diazepam and nordiazepam is determined using 0.5 ml of plasma extracted with 1.0 ml of benzene containing 25 ng/ml of methylnitrazepam as the internal standard. The benzene extract is removed and an aliquot is subjected to automated GLC—ECD analysis. The method has a sensitivity limit of 5 ng diazepam and 10 ng nordiazepam per milliliter of plasma. The method was used to determine the plasma levels in man following the first 5-mg diazepam dose, as well as during chronic oral administration of 5 mg diazepam three times daily and 15 mg diazepam once a day.
ISSN:0378-4347
DOI:10.1016/S0378-4347(00)81313-4