GSH2, a gene encoding γ-glutamylcysteine synthetase in the methylotrophic yeast Hansenula polymorpha

• Published in: FEMS yeast research Vol. 2; no. 3; pp. 327 - 332
• Main Authors: Ubiyvovk, Vira M, Nazarko, Taras Y, Stasyk, Olena G, Sohn, Min Jeong, Kang, Hyun Ah, Sibirny, Andrei A
• Format: Journal Article
• Language: English
• Published: Oxford, UK Elsevier B.V 01.08.2002; Blackwell Publishing Ltd
• Subjects: Amino Acid Sequence; Blotting, Southern; Cloning, Molecular; Genes, Fungal; Genetic Complementation Test; Glutathione - biosynthesis; Glutathione - deficiency; Glutathione Synthase - genetics; Glutathione Synthase - metabolism; Hansenula polymorpha; Methanol - metabolism; Methylotrophic yeast; Molecular Sequence Data; Mutation; Pichia - enzymology; Pichia - genetics; Pichia - growth & development; Sequence Alignment; γ-Glutamylcysteine synthetase; Methylotrophic yeast; γ-Glutamylcysteine synthetase; Glutathione biosynthesis; Hansenula polymorpha
• ISSN: 1567-1356; 1567-1364
• DOI: 10.1016/S1567-1356(02)00111-3

The GSH2 gene, encoding Hansenula polymorpha γ-glutamylcysteine synthetase, was cloned by functional complementation of a glutathione (GSH)-deficient gsh2 mutant of H. polymorpha. The gene was isolated as a 4.3-kb XbaI fragment that was capable of restoring GSH synthesis, heavy-metal resistance and cell proliferation when introduced into gsh2 mutant cells. It possesses 53% identical and 69% similar amino acids compared with the Candida albicans homologue (Gcs1p). In comparison to the Saccharomyces cerevisiae homologue (Gsh1p), it possesses 47% identical and 61% similar amino acids. The GSH2 sequence appears in the GenBank database under accession No. AF435121.