Preparation and characterization of a novel nanocomposite with double enzymes immobilized on magnetic Fe3O4-chitosan-sodium tripolyphosphate

• Published in: Colloids and surfaces, B, Biointerfaces Vol. 169; pp. 280 - 288
• Main Authors: Chen, Zhongqin, Wang, Xiuming, Chen, Yue, Xue, Zihan, Guo, Qingwen, Ma, Qiqi, Chen, Haixia
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.09.2018
• Subjects: Alcalase; Characterization; Chitosan; Enzyme immobilization; Fe3O4 magnetic nanocomposite; Trypsin; Characterization; Trypsin; Fe3O4 magnetic nanocomposite; Enzyme immobilization; Chitosan; Alcalase
• ISSN: 0927-7765; 1873-4367
• DOI: 10.1016/j.colsurfb.2018.04.066

[Display omitted] •A new functionalized magnetic nanocomposite (Fe3O4@CS-TPP) was prepared.•Double enzymes had been efficiently immobilized on Fe3O4@CS-TPP (DE-Fe3O4@CS-TPP MNPs).•DE-Fe3O4@CS-TPP MNPs showed favorable thermal stability, storage stability and reusability.•DE-Fe3O4@CS-TPP MNPs could be efficiently used for food protein hydrolysis. In this study, a novel and efficient synchronously immobilization of double enzymes (DE, alcalase and trypsin) were developed by using the chitosan and sodium tripolyphosphate-coated magnetic nanoparticles (Fe3O4@CS-TPP) as support. The physicochemical properties of DE-Fe3O4@CS-TPP NPs were characterized by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), transmission electron microscopy (TEM) and magnetic measurements. The preparation conditions and stabilities were also optimized and assessed. Results showed that the optimum preparation conditions were as follows: ratio of alcalase and trypsin of 3:1, enzyme concentration of 6% (w/w), temperature of 50 °C and pH of 9.0, the enzyme concentration absorbed by Fe3O4@CS-TPP NPs of 6 mg mL−1, immoblization time of 4 h, and glutaraldehyde content of 3% (w/w). The Km and Vmax values were determined as 0.5035 mg mL−1, 6.900 mg mL−1 min−1 for the immobilized enzymes, respectively. DE-Fe3O4@CS-TPP NPs was more stable than the free enzyme above 40 °C. The activity of DE-Fe3O4@CS-TPP NPs was preserved 86% after 35 days storage and retained more than 60% of its initial activity after ten times of successive reuse. Application of DE-Fe3O4@CS-TPP NPs in various protein hydrolysis showed favorable degrees of hydrolysis, yields and antioxidant activity. The results demonstrated that the DE-Fe3O4@CS-TPP NPs were suitable for application in food protein hydrolysis.