Studies on thermal adaptation of Tetrahymena lipids : Alteration in fatty acid composition and its mechanism in the growth temperature shift-up
A rise in the growth temperature of Tetrahymena pyriformis strain NT-1 from 15 to 34°C resulted in changes in the fatty acid composition of membrane lipids toward higher saturation after a shift-up. The proportion of palmitate (16:0) increased and γ-linolenate (18:3 (6,9,12)) decreased. Alteration i...
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Published in | Biochimica et biophysica acta. Lipids and lipid metabolism Vol. 752; no. 1; pp. 30 - 37 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
Elsevier B.V
01.01.1983
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Subjects | |
Online Access | Get full text |
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Summary: | A rise in the growth temperature of
Tetrahymena pyriformis strain NT-1 from 15 to 34°C resulted in changes in the fatty acid composition of membrane lipids toward higher saturation after a shift-up. The proportion of palmitate (16:0) increased and γ-linolenate (18:3 (6,9,12)) decreased. Alteration in phospholipid composition was completed by a marked increase in phosphatidylethanolamine, with a compensatory decrease in 2-aminoethylphosphonolipid. However, the level of phosphatidylcholine remained unchanged. The rate of incorporation of [1-
14C]acetate into saturated fatty acids (myristate, 14:0; palmitate; stearate, 18:0) was maximum at 1 h after a shift, while formation of
14C-labeled unsaturated fatty acids (palmitoleate, 16:1 (9); linoleate, 18:2 (9,12); γ-linolenate) decreased by about 50%. These findings could be explained by the reduced desaturase activities which were due principally to the decrease in the terminal component, cyanide-sensitive factor (CSF), of the microsomal desaturase system. Furthermore, changes induced by temperature shift-up in activities of microsomal reductases and the content of cytochrome
b
5 were observed to behave comparably with changes in activities of desaturases. |
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ISSN: | 0005-2760 1879-145X |
DOI: | 10.1016/0005-2760(83)90229-1 |