GAP1 activity is dependent on cAMP in Saccharomyces cerevisiae

General amino acid permease (GAP1) activity was evaluated in adenylate cyclase-deficient Saccharomyces cerevisiae to determine the effect of cAMP levels on GAP1 activity. Lowering cAMP concentrations in the culture media led to a decrease in the initial rates of l-citrulline uptake. Kinetics of the...

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Bibliographic Details
Published inFEMS microbiology letters Vol. 151; no. 2; pp. 131 - 133
Main Authors Amitrano, Adrian A, Saenz, Daniel A, Ramos, Eugenia H
Format Journal Article
LanguageEnglish
Published Elsevier B.V 15.06.1997
Subjects
Amino acid transport
cAMP regulation
Saccharomyces cerevisiae
cAMP: adenosine 3′,5′-cyclic monophosphate
Amino acid transport
NPR1: nitrogen-permease reactivation
cAMP regulation
GAP1: general amino acid permease
PKA: cAMP-protein kinase
Saccharomyces cerevisiae
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Summary:General amino acid permease (GAP1) activity was evaluated in adenylate cyclase-deficient Saccharomyces cerevisiae to determine the effect of cAMP levels on GAP1 activity. Lowering cAMP concentrations in the culture media led to a decrease in the initial rates of l-citrulline uptake. Kinetics of the amino acid transport system showed a partial loss of transport capacity, with no apparent modifications in permease affinity.
ISSN:0378-1097
1574-6968
DOI:10.1016/S0378-1097(97)00144-4