Performance of the two-site immunoradiometric assay for serum thyroid-stimulating hormone. Effects of changes in solid-phase matrix and antibody coupling chemistry

Ten solid-phases were evaluated for their usefulness in a two-site immunoradiometric assay for serum thyroid-stimulating hormone. The criteria used to assess each reagent included the minimum detection limit attainable, the change of binding over the concentration range (signal: noise ratio), and ea...

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Bibliographic Details
Published inJournal of immunological methods Vol. 104; no. 1-2; p. 87
Main Authors McConway, M G, Biggart, E M, Chapman, R S
Format Journal Article
LanguageEnglish
Published Netherlands 23.11.1987
Subjects
Antibodies, Monoclonal
Humans
Immunochemistry
Immunosorbent Techniques
Radioimmunoassay - methods
Thyrotropin - analysis
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Summary:Ten solid-phases were evaluated for their usefulness in a two-site immunoradiometric assay for serum thyroid-stimulating hormone. The criteria used to assess each reagent included the minimum detection limit attainable, the change of binding over the concentration range (signal: noise ratio), and ease of preparation of the reagents. Of all the solid phases tested, Sepharose CL-4B and activated CH-Sepharose 4B showed the characteristics best suited to IRMA, while epoxy-activated Sepharose 6B and Ultrogel ACA-44 were marginally less effective.
ISSN:0022-1759
DOI:10.1016/0022-1759(87)90491-1