Suppressive effect of X-irradiated tumor cell presensitization on the induction of syngeneic tumor immunity: II. Opposite effects of intravenous administration of TNP-conjugated tumor cells on the development of antitumor and anti-TNP-self cytotoxic effector cells

• Published in: Cellular immunology Vol. 59; no. 1; pp. 181 - 186
• Main Authors: Fujiwara, Hiromi, Shearer, Gene M.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Inc 15.03.1981
• Subjects: Animals; Antibodies, Neoplasm - immunology; Cytotoxicity, Immunologic; Female; Mice; Mice, Inbred C3H; Nitrobenzenes - immunology; Plasmacytoma - immunology; T-Lymphocytes - immunology; Trinitrobenzenes - immunology; X-Rays
• ISSN: 0008-8749; 1090-2163
• DOI: 10.1016/0008-8749(81)90445-7

A comparison was made of the effects of iv inoculation of trinitrophenyl (TNP)-conjugated syngeneic X5563 tumor cells on the development of anti-TNP-modified self (TNP-self) and anti-X5563 tumor-specific T-cell-mediated cytotoxic responses. Administration of syngeneic TNP-conjugated spleen cells or X-irradiated TNP-conjugated X5563 cells resulted in an appreciable augmentation in the generation of TNP-reactive cytotoxic effector cell activity as measured by subsequent in vitro sensitization with TNP-self. In contrast, iv inoculation with either TNP-modified or unmodified X-irradiated X5563 tumor cells did not prime mice for antitumor cytotoxic responses, but abolished the ability of mice to develop antitumor cytotoxic effector cells even after effective in vivo immunization with tumor cells. The possible mechanisms by which the suppression of antitumor cytotoxic response was induced by conditions which simultaneously induced an enhanced anti-TNP-self cytotoxic response are discussed.