Comparison of paraquat-specific murine monoclonal antibodies produced by in vitro and in vivo immunization

• Published in: Fundamental and applied toxicology Vol. 11; no. 2; pp. 261 - 267
• Main Authors: Johnston, Shane C., Bowles, Mark, Winzor, Donald J., Pond, Susan M.
• Format: Journal Article
• Language: English
• Published: Boston, MA Elsevier Science (USA) 01.08.1988; San Diego, CA Academic Press; New York, NY
• Subjects: Animals; Antibodies, Monoclonal - biosynthesis; Antibodies, Monoclonal - isolation & purification; Antibody Specificity; ANTICORPS MONOCLONAL; ANTICUERPOS MONOCLONALES; Biological and medical sciences; Cell Fusion; Chromatography, High Pressure Liquid; CLONACION; CLONAGE; CLONING; Cloning, Molecular; Cross Reactions; Enzyme-Linked Immunosorbent Assay; EPURATION; EXPERIMENTACION IN VITRO; EXPERIMENTACION IN VIVO; EXPERIMENTATION IN VITRO; EXPERIMENTATION IN VIVO; HERBICIDAS; HERBICIDE; HERBICIDES; IMMUNISATION; IMMUNIZATION; Immunoglobulin G - immunology; Immunoglobulin M - immunology; IN VITRO EXPERIMENTATION; IN VIVO EXPERIMENTATION; INMUNIZACION; Medical sciences; Mice; Mice, Inbred BALB C; MONOCLONAL ANTIBODIES; Paraquat - immunology; Pesticides, fertilizers and other agrochemicals toxicology; PURIFICACION; PURIFICATION; Thymus Gland - cytology; Thymus Gland - drug effects; Toxicology; Antibody; Rodentia; Pesticides; Hybridoma; Monoclonal antibody; In vitro; Herbicide; Active immunization; Quaternary ammonium compound; In vivo; Vertebrata; Mammalia; Treatment; Mouse; Animal; Immunotherapy; Production; Poisoning
• ISSN: 0272-0590; 1095-6832
• DOI: 10.1016/0272-0590(88)90150-9

The techniques of in vitro and in vivo immunization have been used to produce murine hybridomas secreting anti-paraquat IgM and IgG antibodies, respectively. The monoclonal antibodies from these IgM-secreting and IgG-secreting cell lines have been purified by HPLC and characterized by ELISA for their affinities to eliciting antigen and cross-reactivity. Both antibody preparations exhibited high selectivity and similar affinities for paraquat. There was only a minor degree of interaction with a range of paraquat analogs that differed in the nature and position of group substituents.