The cellular basis of adjuvant arthritis: I. Enhancement of cell-mediated passive transfer by concanavalin A and by immunosuppressive pretreatment of the recipient

• Published in: Cellular immunology Vol. 75; no. 2; pp. 271 - 282
• Main Authors: Taurog, Joel D., Sandberg, Gregory P., Mahowald, Maren L.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Inc 01.02.1983
• Subjects: Animals; Arthritis - immunology; Arthritis, Experimental - immunology; Concanavalin A - pharmacology; Graft vs Host Reaction; Immune Tolerance; Immunity, Cellular; Immunization, Passive; Lymph Nodes - cytology; Lymph Nodes - radiation effects; Lymphocyte Activation; Male; Mice; Rats; Rats, Inbred Lew; Spleen - cytology; Spleen - radiation effects; T-Lymphocytes - immunology
• ISSN: 0008-8749; 1090-2163
• DOI: 10.1016/0008-8749(83)90325-8

Two reliable systems for the cell-mediated passive transfer of adjuvant arthritis have been developed. Donor rats were sensitized with Mycobacterium butyricum in mineral oil. In the first system, intravenous injection of adjuvant-sensitized donor lymph node or spleen cells into adultthymectomized, lethally irradiated, bone marrow cell-reconstituted syngeneic rats induced arthritis in the recipients. In the second system, adjuvant-sensitized donor lymph node or spleen cells were cultured in vitro with concanavalin A; these cells induced arthritis in normal recipients as well as in thymectomized, irradiated, bone marrow cell-reconstituted recipients. The passively transferred disease in both systems resembled classical adjuvant-induced arthritis clinically, radiographically, and histologically. Neither irradiated, adjuvant-sensitized donor cells nor cells from donors not injected with complete adjuvant could passively transfer arthritis.