Dietary regulation of glycolytic enzymes: XI. Effect of inhibitors of protein synthesis on the adaptation of certain jejunal glycolytic and folate-metabolizing enzymes to diet and sex steroids

• Published in: Biochimica et biophysica acta Vol. 237; no. 3; pp. 484 - 489
• Main Authors: Stifel, Fred B., Herman, Robert H., Rosensweig, Norton S.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 22.06.1971
• Subjects: Aldehyde-Lyases - biosynthesis; Animals; Caseins - pharmacology; Dactinomycin - pharmacology; Dietary Carbohydrates; Dietary Proteins; Enzyme Induction; Estradiol - pharmacology; Ethionine - pharmacology; Fasting; Female; Folic Acid; Fructose - pharmacology; Fructosephosphates; Glycolysis - drug effects; Jejunum - drug effects; Jejunum - enzymology; Male; Metabolism - drug effects; NAD; Oxidoreductases - biosynthesis; Pyruvate Kinase - biosynthesis; Rats; RNA - biosynthesis; Serine; Testosterone - pharmacology; Time Factors; Transferases - biosynthesis
• ISSN: 0304-4165; 0006-3002; 1872-8006
• DOI: 10.1016/0304-4165(71)90267-4

1. 1. The effects of oral sex hormones, dietary fructose and casein without and with actinomycin D or ethionine and fasting upon the activities of two folate-metabolizing enzymes (serine hydroxymethyltransferase ( L-Serine: tetrahydrofolate 5,10-hydroxymethyltransferase, E.C. 2.1.2.1) and methylenetetrahydrofolate dehydrogenase (5,10-methylenetetrahydrofolate: NADP oxidoreductase, E.C. 1.5.1.5)) and two glycolytic enzymes (pyruvate kinase (ATP: pyruvate phosphotransferase, E.C. 2.7.1.40) and fructose diphosphate aldolase (fructose-1,6-diphosphate D-glyceraldehyde-3-phosphate lyase, E.C. 4.1.2.13)) were studied in the jejunum of female and male rats. 2. 2. Actinomycin, an inhibitor of DNA-dependent RNA synthesis, and ethionine, an inhibitor of protein synthesis, both effectively decreased the adaptive responses of jejunal glycolytic and folate-metabolizing enzymes to diet and oral sex hormones. These findings lend support to the hypothesis that stimulation of synthesis of certain RNA species is probably one of the primary mechanisms by which sex hormones (17β -estradiol and testosteron) and diet produce adaptive increases in the activities of certain jejunal glycolytic and folate-metabolizing enzymes.