OsWRKY12 negatively regulates the drought-stress tolerance and secondary cell wall biosynthesis by targeting different downstream transcription factor genes in rice

• Published in: Plant physiology and biochemistry Vol. 212; p. 108794
• Main Authors: Jia, Shuzhen, Wang, Chunyue, Sun, Wanying, Yan, Xiaofei, Wang, Weiting, Xu, Bing, Guo, Guangyan, Bi, Caili
• Format: Journal Article
• Language: English
• Published: Elsevier Masson SAS 01.07.2024
• Subjects: Drought stress; Negative; Rice; Secondary cell wall; WRKY; Drought stress; WRKY; Negative; Secondary cell wall; Rice
• ISSN: 0981-9428; 1873-2690; 1873-2690
• DOI: 10.1016/j.plaphy.2024.108794

With the increasing occurrence of global warming, drought is becoming a major constraint for plant growth and crop yield. Plant cell walls experience continuous changes during the growth, development, and in responding to stressful conditions. The plant WRKYs play pivotal roles in regulating the secondary cell wall (SCW) biosynthesis and helping plant defend against abiotic stresses. qRT-PCR evidence showed that OsWRKY12 was affected by drought and ABA treatments. Over-expression of OsWRKY12 decreased the drought tolerance of the rice transgenics at the germination stage and the seedling stage. The transcription levels of drought-stress-associated genes as well as those genes participating in the ABA biosynthesis and signaling were significantly different compared to the wild type (WT). Our results also showed that less lignin and cellulose were deposited in the OsWRKY12-overexpressors, and heterogenous expression of OsWRKY12 in atwrky12 could lower the increased lignin and cellulose contents, as well as the improved PEG-stress tolerance, to a similar level as the WT. qRT-PCR results indicated that the transcription levels of all the genes related to lignin and cellulose biosynthesis were significantly decreased in the rice transgenics than the WT. Further evidence from yeast one-hybrid assay and the dual-luciferase reporter system suggested that OsWRKY12 could bind to promoters of OsABI5 (the critical component of the ABA signaling pathway) and OsSWN3/OsSWN7 (the key positive regulators in the rice SCW thickening), and hence repressing their expression. In conclusion, OsWRKY12 mediates the crosstalk between SCW biosynthesis and plant stress tolerance by binding to the promoters of different downstream genes. •OsWRKY12 negatively regulates the drought-stress response by affecting the ABA biosynthesis and signaling pathway.•Rice seedlings over-expressing OsWRKY12 showed decreased secondary cell wall (SCW) biosynthesis.•OsWRKY12 mediates the crosstalk of the drought-stress response and SCW biosynthesis by targeting to different transcription factor genes.