Analysis of sisomicin binding sites in Micromonospora inyoensis cell wall

Sisomicin binding sites are located in the cell wall. They are the carboxyl groups of peptidoglycans, which are major components of the cell wall. The carboxyl groups have negative charges which can bind the positively charged amino groups of sisomicin. When the negative charges of the carboxyl grou...

Full description

Saved in:
Bibliographic Details
Published inFEMS microbiology letters Vol. 166; no. 1; pp. 9 - 13
Main Authors Kim, Dae Ho, Suh, Jung Hae, Ju, Jae Yeong, Yum, Sung Kwan, Shin, Chul Soo
Format Journal Article
LanguageEnglish
Published Elsevier B.V 01.09.1998
Subjects
Cell wall binding site
Micromonospora inyoensis
Sisomicin
Sisomicin
Cell wall binding site
Micromonospora inyoensis
Online AccessGet full text

Cover

More Information
Summary:Sisomicin binding sites are located in the cell wall. They are the carboxyl groups of peptidoglycans, which are major components of the cell wall. The carboxyl groups have negative charges which can bind the positively charged amino groups of sisomicin. When the negative charges of the carboxyl groups of the peptidoglycans are changed to neutral or positive charges, sisomicin does not bind to the cell wall. Magnesium ions bind to the cell wall in competition with sisomicin, and have a weak affinity for the cell wall binding sites compared with sisomicin. The binding molar ratio of sisomicin to magnesium ions was approximately 1 to 10.
ISSN:0378-1097
1574-6968
DOI:10.1016/S0378-1097(98)00304-8