Coaggregation between Prevotella nigrescens and Prevotella intermedia with Actinomyces naeslundii strains

Using a visual coaggregation assay, 43% (6 of 14) of Prevotella nigrescens and 50% (4 of 8) of Prevotella intermedia strains coaggregated with Actinomyces naeslundii strains which represented the six Actinomyces coaggregation groups (A to F). For both species, coaggregation occurred most frequently...

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Bibliographic Details
Published inFEMS microbiology letters Vol. 132; no. 3; pp. 291 - 296
Main Authors Cookson, Adrian L., Handley, Pauline S., Jacob, Alan E., Watson, G.Keith, Allison, Clive
Format Journal Article
LanguageEnglish
Published Elsevier B.V 15.10.1995
Subjects
Actinomyces naeslundii
Aggregation
Interbacterial coaggregation
Prevotella intermedia
Prevotella nigrescens
Actinomyces naeslundii
Aggregation
Prevotella nigrescens
Interbacterial coaggregation
Prevotella intermedia
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Summary:Using a visual coaggregation assay, 43% (6 of 14) of Prevotella nigrescens and 50% (4 of 8) of Prevotella intermedia strains coaggregated with Actinomyces naeslundii strains which represented the six Actinomyces coaggregation groups (A to F). For both species, coaggregation occurred most frequently with A. naeslundii strains from coaggregation groups C, D and E. No coaggregation was observed with Actinomyces israelii, Actinomyces odontolyticus or six oral Streptococcus species. Coaggregation was not inhibited by lactose, saliva or serum. Pretreatment of Prevotella strains with heat, SDS and proteinase K abolished coaggregation when the treated cells were added to untreated Actinomyces strains. The same pretreatment of the Actinomyces strains had no effect on their ability to coaggregate with untreated Prevotella strains. Pretreatment of all coaggregating P. nigrescens strains with trypsin abolished coaggregation, whereas the coaggregation ability of the P. intermedia and Actinomyces strains was resistant to trypsin pretreatment. Pretreatment of the strains of both Prevotella species and the Actinomyces with periodate abolished coaggregation in all cases. These results suggest that the Prevotella strains each possess a protein coaggregation adhesin, which for the P. intermedia strains is resistant to trypsin, that interacts with a non-protein receptor on the A. naeslundii strains.
ISSN:0378-1097
1574-6968
DOI:10.1016/0378-1097(95)00331-X