K 3 killer yeast is a mutant K 2 killer yeast

The K 2 and K 3 killer yeast classes in Saccharomyces cerevisiae were compared. Sensitive strain 381 and the killer strains NCYC 232 NCYC 190, NCYC 738, NCYC 761 and KT28 were sensitive to the killer toxins produced by the K 2 and K 3 strains. However, these two strains were resistant to both toxins...

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Published inMycological research Vol. 94; no. 7; pp. 901 - 906
Main Authors Wingfield, Brenda D., Van Der Meer, Linda J., Pretorius, Isak S., Van Vuuren, Hendrik J.J.
Format Journal Article
LanguageEnglish
Published Elsevier Ltd 01.10.1990
Subjects
dsRNA
Killer yeast
Saccharomyces cerevisiae
dsRNA
Saccharomyces cerevisiae
Killer yeast
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Summary:The K 2 and K 3 killer yeast classes in Saccharomyces cerevisiae were compared. Sensitive strain 381 and the killer strains NCYC 232 NCYC 190, NCYC 738, NCYC 761 and KT28 were sensitive to the killer toxins produced by the K 2 and K 3 strains. However, these two strains were resistant to both toxins. Hybridization analysis showed that the M dsRNAs from the K 2 and K 3 strains had significant homology, but shared no homology with the dsRNAs from the K 1 and KT28 strains. The K 2 M dsRNA is larger than the K 3 M dsRNA, and electron micrographs showed an unmatched loop when these molecules were hybridized. This suggests that the K 3 M dsRNA is a mutant K 2 M genome. These results show that K 2 and K 3 strains belong in the same class, but separate from the K 1 and KT28 killer ty
ISSN:0953-7562
1469-8102
DOI:10.1016/S0953-7562(09)81304-X