Coincidence studies of O 2−, O − and electron formation in electron-stripping of cationic biomolecules by molecular oxygen

In high-energy collisions between protonated biomolecules (amino acids, peptides and proteins) and molecular oxygen, electron-stripping of the cations occurs, i.e., they increase their charge state by one unit. The fate of the stripped electron depends on the cation under study. We have measured the...

Full description

Saved in:
Bibliographic Details
Published inInternational journal of mass spectrometry Vol. 214; no. 1; pp. 57 - 62
Main Authors Tomita, Shigeo, Forster, James S, Hvelplund, Preben, Nielsen, Steen Brøndsted
Format Journal Article
LanguageEnglish
Published Elsevier B.V 15.02.2002
Subjects
Biomolecules
Coincidence studies
Electron-stripping
High-energy collisions
Electron-stripping
Biomolecules
High-energy collisions
Coincidence studies
Online AccessGet full text

Cover

More Information
Summary:In high-energy collisions between protonated biomolecules (amino acids, peptides and proteins) and molecular oxygen, electron-stripping of the cations occurs, i.e., they increase their charge state by one unit. The fate of the stripped electron depends on the cation under study. We have measured the negative recoils coincident with the electron-stripped ions to be O 2 −, O −, and a free electron (which might be a result of autodetachment from an excited state of O 2 −). The ratio of the O 2 − recoils to electrons decreases in the following order: [ Lys+9 H] 10+>[ Ubi+7 H] 8+>[ GS+2 H] 3+> TyrH 2+, PheH 2+ , in accordance with decreasing interaction times (Lys: lysozyme, Ubi: ubiquitine, GS: gramicidin S, Tyr: tyrosine, Phe: phenylalanine).
ISSN:1387-3806
1873-2798
DOI:10.1016/S1387-3806(01)00555-3