Copper-zinc superoxide dismutase and isolectin B 4 binding are markers for associative and transhemispheric diaschisis induced by focal ischemia in rat cortex

Copper/zinc-superoxide dismutase (Cu/Zn-SOD) belongs to a class of enzymes, identified as essential and highly effective endogenous scavengers of cytotoxic oxygen radicals. These radicals contribute to postlesional neurotoxicity. In order to determine the superoxide-scavenging potential of regions a...

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Published inNeuroscience letters Vol. 228; no. 3; pp. 163 - 166
Main Authors Bidmon, Hans-J, Oermann, Evelyn, Schleicher, Axel, Kato, Kanefusa, Kinscherf, Ralf, Buchkremer-Ratzmann, Irmgard, Witte, Otto W, Zilles, Karl
Format Journal Article
LanguageEnglish
Published Elsevier Ireland Ltd 13.06.1997
Subjects
Diaschisis
Free radicals
Microglia
Photothrombosis
Projection neurons
Stroke
Superoxide dismutase
Thalamus
Projection neurons
Stroke
Free radicals
Superoxide dismutase
Thalamus
Diaschisis
Photothrombosis
Microglia
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Summary:Copper/zinc-superoxide dismutase (Cu/Zn-SOD) belongs to a class of enzymes, identified as essential and highly effective endogenous scavengers of cytotoxic oxygen radicals. These radicals contribute to postlesional neurotoxicity. In order to determine the superoxide-scavenging potential of regions affected by unilateral cortical photothrombosis, we studied the changes in the distribution of Cu/Zn-SOD and the appearance of activated microglia by immunohistochemistry and isolectin B 4 binding. Four hours postlesion, Cu/Zn-SOD increased significantly within a homotopic area of the contralateral hemisphere and in ipsilateral thalamic nuclei, whereas isolectin B 4-positive microglia were upregulated at days 5 and 7 postlesion within the same regions. The contralateral increase in the amount of the superoxide-scavenging Cu/Zn-SOD indicates that this enzyme is induced by a retrograde reaction carried through callosal connections.
ISSN:0304-3940
1872-7972
DOI:10.1016/S0304-3940(97)00389-3