Interactive effects of methyl-deficiency and dietary restrictionon liver cell proliferation and telomerase activity in Fischer 344 rats pretreated with aflatoxin B 1
The effects of methyl-deficiency and dietary restriction (DR) on hepatic cell proliferation and telomerase activity was studied in male Fischer 344 rats pretreated with aflatoxin B 1 (AFB 1). Five-week-old rats were gavaged 5 days per week for 3 weeks with AFB 1 (25 μg/rat per day) or solvent (100 μ...
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Published in | Cancer letters Vol. 152; no. 1; pp. 53 - 61 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
Elsevier Ireland Ltd
28.04.2000
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Subjects | |
Online Access | Get full text |
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Summary: | The effects of methyl-deficiency and dietary restriction (DR) on hepatic cell proliferation and telomerase activity was studied in male Fischer 344 rats pretreated with aflatoxin B
1 (AFB
1). Five-week-old rats were gavaged 5 days per week for 3 weeks with AFB
1 (25 μg/rat per day) or solvent (100 μl 75% dimethylsulfoxide). Rats were then divided into four groups. Two groups were fed a methyl-sufficient (MS) diet either ab libitum (AL) or with DR. The other two groups were fed a methyl-deficient (MD) diet either AL or with DR. At 15, 20, and 32 weeks of age, hepatic cell proliferation, telomerase activity, and the number of glutathione
S-transferase-P positive (GST-P
+) foci were determined. DR reduced hepatic cell proliferation, while the MD diet and AFB
1 pretreatment increased cell proliferation. Telomerase activity was decreased by DR and increased by the MD diet and AFB
1 pretreatment. The same trend was observed with GST-P
+ foci: in AFB
1-pretreated rats, methyl deficiency increased the number of foci, while DR decreased the number. These results are consistent with a role of telomerase in hepatocarcinogenesis. |
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ISSN: | 0304-3835 1872-7980 |
DOI: | 10.1016/S0304-3835(99)00436-X |