Oxidation of low-density lipoprotein by Cu 2+ and lipoxygenase: an electron spin resonance study

The aim of this work was to obtain spectroscopic evidence for free radicals formed during copper ion- and lipoxygenase-catalyzed oxidation of the low-density lipoprotein. During the initial oxidation phase, a free-radical metabolite derived from the endogeneous α-tocopherol present in the low-densit...

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Bibliographic Details
Published inBiochimica et biophysica acta. General subjects Vol. 1035; no. 3; pp. 286 - 292
Main Authors Kalyanaraman, B., Antholine, William E., Parthasarathy, Sampath
Format Journal Article
LanguageEnglish
Published Elsevier B.V 14.09.1990
Subjects
Copper ion
ESR
Free radical
Lipoxygenase
Low density lipoprotein
Oxidation
PBS
Copper ion
ESR
LDL
Oxidation
Lipoxygenase
Low density lipoprotein
PBN
PLA 2
Free radical
MNP
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Summary:The aim of this work was to obtain spectroscopic evidence for free radicals formed during copper ion- and lipoxygenase-catalyzed oxidation of the low-density lipoprotein. During the initial oxidation phase, a free-radical metabolite derived from the endogeneous α-tocopherol present in the low-density lipoprotein was detected by the electron spin resonance technique. The divalent copper ions were bound to the residual EDTA present in the low-density lipoprotein and to the protein. Production of the α-tocopherol radical was suppressed in the presence of spin traps. Evidence for the low-density lipoprotein-lipid derived radicals was obtained by ESR-spin trapping methods. Implications of these findings in the oxidative modification of the low-density lipoprotein are discussed.
ISSN:0304-4165
1872-8006
DOI:10.1016/0304-4165(90)90090-J